Optimization of Extraction Temperature and Time for Antioxidant Lentinus Edodes using Response Surface Methodology

Rahayu Ahamd; Aisyah Mardhiyah Abd Ghafar; Silambarasi Mooralitharan; Imtinan Raihana Alfadino Akbar; Nurul Farihan Mohamed

doi:10.37934/sijpce.1.1.1525a

Authors

Rahayu Ahamd Al Razi Halal Action Laboratory, Kolej PERMATA Insan, Universiti Sains Islam Malaysia, 71800 Nilai, Negeri Sembilan, Malaysia
Aisyah Mardhiyah Abd Ghafar Al Razi Halal Action Laboratory, Kolej PERMATA Insan, Universiti Sains Islam Malaysia, 71800 Nilai, Negeri Sembilan, Malaysia
Silambarasi Mooralitharan Al Razi Halal Action Laboratory, Kolej PERMATA Insan, Universiti Sains Islam Malaysia, 71800 Nilai, Negeri Sembilan, Malaysia
Imtinan Raihana Alfadino Akbar Al Razi Halal Action Laboratory, Kolej PERMATA Insan, Universiti Sains Islam Malaysia, 71800 Nilai, Negeri Sembilan, Malaysia
Nurul Farihan Mohamed Al Razi Halal Action Laboratory, Kolej PERMATA Insan, Universiti Sains Islam Malaysia, 71800 Nilai, Negeri Sembilan, Malaysia

DOI:

https://doi.org/10.37934/sijpce.1.1.1525a

Keywords:

Lentinus edodes, extraction, Response Surface Methodology (RSM), Central Composite Design (CCD)

Abstract

The extraction conditions influencing the yield of crude polysaccharide and antioxidant activity in *Lentinus edodes* were studied and optimized using response surface methodology (RSM). Central composite design (CCD) was applied to optimize the extraction time and temperature. The results showed that the data were well-fitted to second-order polynomial models. The independent variables, temperature (A) and time (B), had significant linear, quadratic, and interaction effects on total carbohydrate content (TCC), total flavonoid content (TFC), and DPPH radical-scavenging activity. ANOVA analysis revealed that the models were highly significant (p < 0.001) for TCC, TFC, and DPPH radical-scavenging activity. The optimal extraction conditions were 225 minutes at 65°C, producing 55 mg/mL of carbohydrate content (TCC); 420 minutes at 100°C, yielding 53 mg/mL of flavonoid content (TFC); and 225 minutes at 65°C, resulting in 75.5% DPPH radical-scavenging activity. The models were validated, and the experimental values exceeded the predicted optimal values, reaching 66 mg/mL, 67 mg/mL, and 84.6% for TCC, TFC, and DPPH radical-scavenging activity, respectively.